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Product Info Summary
|Description:||Transient overexpression lysate of retinoblastoma binding protein 4 (RBBP4), transcript variant 1|
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RbAp48 (RBBP4) (NM_005610) Human Over-expression Lysate
Transient overexpression lysate of retinoblastoma binding protein 4 (RBBP4), transcript variant 1
Storage & Handling
Cite This Product
RbAp48 (RBBP4) (NM_005610) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005928)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RbAp48 (RBBP4) Human Over-expression Lysates NM_005610.
Western blot validation of overexpression lysate (LS005928) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RBBP4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RBBP4 (Source: Uniprot.org, NCBI)
Histone-binding protein RBBP4
WD repeat RBAP46/RBAP48/MSI1 family
CAF-1 p48; CAF-1 subunit C; CAF-1C; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; NURF55; RBAP48; RBBP4; RBBP-4; retinoblastoma binding protein 4; Retinoblastoma-binding protein 4CAF-I 48 kDa subunit; Retinoblastoma-binding protein p48 RBBP4 NURF55, RBAP48, lin-53 RB binding protein 4, chromatin remodeling factor histone-binding protein RBBP4|CAF-1 subunit C|CAF-I 48 kDa subunit|CAF-I p48|MSI1 protein homolog|RBBP-4|chromatin assembly factor 1 subunit C|chromatin assembly factor I p48 subunit|chromatin assembly factor/CAF-1 p48 subunit|nucleosome-remodeling factor subunit RBAP48|retinoblastoma-binding protein 4|retinoblastoma-binding protein p48*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RBBP4, check out the RBBP4 Infographic
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In this infographic, you will see the following information for RBBP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS005928
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