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Product Info Summary
|Description:||Transient overexpression lysate of chromosome 7 open reading frame 64 (C7orf64)|
RBM48 (NM_032120) Human Over-expression Lysate
Transient overexpression lysate of chromosome 7 open reading frame 64 (C7orf64)
Storage & Handling
Cite This Product
RBM48 (NM_032120) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084060)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RBM48 Human Over-expression Lysates NM_032120.
Western blot validation of overexpression lysate (LS084060) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RBM48 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RBM48 (Source: Uniprot.org, NCBI)
RNA-binding protein 48
C7orf64; chromosome 7 open reading frame 64; DKFZp564O0523; DKFZp686D1651; HSPC304; hypothetical protein LOC84060; RNA binding motif protein 48 RBM48 C7orf64, HSPC304 RNA binding motif protein 48 RNA-binding protein 48|UPF0712 protein C7orf64*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS084060
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