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Product Info Summary
|Description:||Transient overexpression lysate of RNA binding motif protein 4B (RBM4B)|
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RBM4B (NM_031492) Human Over-expression Lysate
Transient overexpression lysate of RNA binding motif protein 4B (RBM4B)
Storage & Handling
Cite This Product
RBM4B (NM_031492) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083759)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RBM4B Human Over-expression Lysates NM_031492.
Western blot validation of overexpression lysate (LS083759) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RBM4B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RBM4B (Source: Uniprot.org, NCBI)
RNA-binding protein 4B
MGC10871; RBM30; RBM4L; RNA binding motif protein 30; RNA binding motif protein 4B; RNA-binding motif protein 30; RNA-binding motif protein 4B; RNA-binding protein 30; RNA-binding protein 4B; ZCCHC15; ZCRB3B; zinc finger CCHC-type and RNA binding motif 3B RBM4B RBM30, RBM4L, ZCCHC15, ZCCHC21B, ZCRB3B RNA binding motif protein 4B RNA-binding protein 4B|RNA-binding motif protein 30|zinc finger CCHC-type and RNA binding motif 3B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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