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Product Info Summary
|Description:||Transient overexpression lysate of retinoid X receptor, beta (RXRB)|
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Retinoid X Receptor beta (RXRB) (NM_021976) Human Over-expression Lysate
Transient overexpression lysate of retinoid X receptor, beta (RXRB)
Storage & Handling
Cite This Product
Retinoid X Receptor beta (RXRB) (NM_021976) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006257)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Retinoid X Receptor beta (RXRB) Human Over-expression Lysates NM_021976.
Western blot validation of overexpression lysate (LS006257) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RXRB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RXRB (Source: Uniprot.org, NCBI)
Retinoic acid receptor RXR-beta
nuclear hormone receptor family
DAUDI6; H-2RIIBP; MHC class I promoter binding protein; NR2B2; NR2B2MGC1831; Nuclear receptor subfamily 2 group B member 2; RCoR-1; retinoic acid receptor RXR-beta; Retinoid X receptor beta; retinoid X receptor, beta; RXR beta; RXRB RXRB DAUDI6, H-2RIIBP, NR2B2, RCoR-1 retinoid X receptor beta retinoic acid receptor RXR-beta|MHC class I promoter binding protein|nuclear receptor subfamily 2 group B member 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RXRB, check out the RXRB Infographic
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Specific Publications For Retinoid X Receptor beta (RXRB) (NM_021976) Human Over-expression Lysate (LS006257)
No publications found for LS006257
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