RFX4 (NM_032491) Human Over-expression Lysate

Lysate of RFX4 over-expression cell lines, WB control

Transient overexpression lysate of regulatory factor X, 4 (influences HLA class II expression) (RFX4), transcript variant 1

Product Info Summary

SKU: LS005992
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of regulatory factor X, 4 (influences HLA class II expression) (RFX4), transcript variant 1

Product Name

RFX4 (NM_032491) Human Over-expression Lysate

See all RFX4 products

SKU/Catalog Number

LS005992

Size

100 µg

Description

Transient overexpression lysate of regulatory factor X, 4 (influences HLA class II expression) (RFX4), transcript variant 1

Storage & Handling

Cite This Product

RFX4 (NM_032491) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005992)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for RFX4 (Source: Uniprot.org, NCBI)

Uniprot ID

Q33E94

Gene ID

5992

Gene Name

RFX4

Full Name

Transcription factor RFX4

Weight

83.368kDa

Superfamily

RFX family

Alternative Names

NYD-SP10; Regulatory factor X 4; regulatory factor X, 4 (influences HLA class II expression); regulatory factor X4; Testis development protein NYD-SP10; transcription factor NYD-sp10; transcription factor RFX4 RFX4 NYD-SP10 regulatory factor X4 transcription factor RFX4|regulatory factor X, 4 (influences HLA class II expression)|testis development protein NYD-SP10|winged-helix transcription factor RFX4

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on RFX4, check out the RFX4 Infographic

RFX4 infographic

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In this infographic, you will see the following information for RFX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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