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Product Info Summary
|Description:||Transient overexpression lysate of ribosomal protein L29 (RPL29)|
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RPL29 (NM_000992) Human Over-expression Lysate
Transient overexpression lysate of ribosomal protein L29 (RPL29)
Storage & Handling
Cite This Product
RPL29 (NM_000992) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006159)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RPL29 Human Over-expression Lysates NM_000992.
Western blot validation of overexpression lysate (LS006159) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPL29 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RPL29 (Source: Uniprot.org, NCBI)
60S ribosomal protein L29
eukaryotic ribosomal protein eL29 family
60S ribosomal protein L29; Cell surface heparin-binding protein HIP; heparin/heparan sulfate-binding protein; heparin/heparan sulfate-interacting protein; HIPHUMRPL29; HP/HS-interacting protein; MGC88589; ribosomal protein L29 RPL29 HIP, HUMRPL29, L29P10, RPL29_3_370, RPL29 ribosomal protein L29 60S ribosomal protein L29|HP/HS-interacting protein|cell surface heparin-binding protein HIP|heparin/heparan sulfate-binding protein|heparin/heparan sulfate-interacting protein|large ribosomal subunit protein eL29|ribosomal protein YL43 homologue*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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