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Product Info Summary
|Description:||Transient overexpression lysate of ribosomal protein L36a (RPL36A)|
RPL36A (NM_021029) Human Over-expression Lysate
Transient overexpression lysate of ribosomal protein L36a (RPL36A)
Storage & Handling
Cite This Product
RPL36A (NM_021029) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006173)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RPL36A Human Over-expression Lysates NM_021029.
Western blot validation of overexpression lysate (LS006173) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPL36A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RPL36A (Source: Uniprot.org, NCBI)
60S ribosomal protein L36a
eukaryotic ribosomal protein eL42 family
60S ribosomal protein L36a; Cell growth-inhibiting gene 15 protein; Cell migration-inducing gene 6 protein; dJ164F3.3 (ribosomal protein L44); L44L; L44-like ribosomal protein; MGC72020; MIG6,60S ribosomal protein L44; ribosomal protein L36a; ribosomal protein L44; RPL36AL; RPL44 RPL36A L36A, L44L, MIG6, RPL44 ribosomal protein L36a 60S ribosomal protein L36a|60S ribosomal protein L44|L44-like ribosomal protein|cell growth-inhibiting gene 15 protein|cell migration-inducing gene 6 protein|dJ164F3.3 (ribosomal protein L44)|large ribosomal subunit protein eL42*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006173
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