RPS18 (NM_022551) Human Over-expression Lysate

Lysate of RPS18 over-expression cell lines, WB control

Transient overexpression lysate of ribosomal protein S18 (RPS18)

Product Info Summary

SKU: LS006222
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of ribosomal protein S18 (RPS18)

Product Name

RPS18 (NM_022551) Human Over-expression Lysate

See all RPS18 products

SKU/Catalog Number

LS006222

Size

100 µg

Description

Transient overexpression lysate of ribosomal protein S18 (RPS18)

Storage & Handling

Cite This Product

RPS18 (NM_022551) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006222)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for RPS18 (Source: Uniprot.org, NCBI)

Uniprot ID

P62269

Gene ID

6222

Gene Name

RPS18

Full Name

40S ribosomal protein S18

Weight

17.719kDa

Superfamily

universal ribosomal protein uS13 family

Alternative Names

D6S218E; HKE3; KE3; KE-3; Ke-3,40S ribosomal protein S18; MGC117351; MGC126835; MGC126837; rhabdomyosarcoma antigen MU-RMS-40.21; ribosomal protein S18 RPS18 D6S218E, HKE3, KE-3, KE3, S18 ribosomal protein S18 40S ribosomal protein S18|rhabdomyosarcoma antigen MU-RMS-40.21|small ribosomal subunit protein uS13

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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