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Product Info Summary
|Description:||Transient overexpression lysate of ribosomal protein S19 (RPS19)|
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RPS19 (NM_001022) Human Over-expression Lysate
Transient overexpression lysate of ribosomal protein S19 (RPS19)
Storage & Handling
Cite This Product
RPS19 (NM_001022) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006223)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RPS19 Human Over-expression Lysates NM_001022.
Western blot validation of overexpression lysate (LS006223) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS19 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RPS19 (Source: Uniprot.org, NCBI)
40S ribosomal protein S19
eukaryotic ribosomal protein eS19 family
DBADBA1,40S ribosomal protein S19; ribosomal protein S19 RPS19 DBA, DBA1, LOH19CR1, S19, eS19 ribosomal protein S19 40S ribosomal protein S19|loss of heterozygosity on chromosome 19, region 1|loss of heterozygosity, 19, chromosomal region 1|small ribosomal subunit protein eS19*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RPS19, check out the RPS19 Infographic
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No publications found for LS006223
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