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Product Info Summary
|Description:||Transient overexpression lysate of ribosomal protein S23 (RPS23)|
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RPS23 (NM_001025) Human Over-expression Lysate
Transient overexpression lysate of ribosomal protein S23 (RPS23)
Storage & Handling
Cite This Product
RPS23 (NM_001025) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006228)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RPS23 Human Over-expression Lysates NM_001025.
Western blot validation of overexpression lysate (LS006228) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS23 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RPS23 (Source: Uniprot.org, NCBI)
40S ribosomal protein S23
universal ribosomal protein uS12 family
FLJ35016; homolog of yeast ribosomal protein S28; ribosomal protein S23,40S ribosomal protein S23 RPS23 BTDD, MABAS, MCINS, PAMAS, S23, uS12 ribosomal protein S23 40S ribosomal protein S23|homolog of yeast ribosomal protein S28|small ribosomal subunit protein uS12*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RPS23, check out the RPS23 Infographic
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In this infographic, you will see the following information for RPS23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS006228
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