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Product Info Summary
|Description:||Transient overexpression lysate of sodium channel, voltage-gated, type II, beta (SCN2B)|
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SCN2B (NM_004588) Human Over-expression Lysate
Transient overexpression lysate of sodium channel, voltage-gated, type II, beta (SCN2B)
Storage & Handling
Cite This Product
SCN2B (NM_004588) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006327)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SCN2B Human Over-expression Lysates NM_004588.
Western blot validation of overexpression lysate (LS006327) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCN2B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SCN2B (Source: Uniprot.org, NCBI)
Sodium channel subunit beta-2
sodium channel auxiliary subunit SCN2B (TC 8.A.17) family
neuronal voltage-gated sodium channel beta 2 subunit; sodium channel beta 2 subunit; sodium channel subunit beta-2; sodium channel, voltage-gated, type II, beta polypeptide; sodium channel, voltage-gated, type II, beta SCN2B ATFB14 sodium voltage-gated channel beta subunit 2 sodium channel subunit beta-2|neuronal voltage-gated sodium channel beta 2 subunit|sodium channel, voltage gated, type II beta subunit|sodium channel, voltage-gated, type II, beta polypeptide*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SCN2B, check out the SCN2B Infographic
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