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Product Info Summary
|Description:||Transient overexpression lysate of sodium channel, nonvoltage-gated 1, delta (SCNN1D), transcript variant 2|
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SCNN1D (NM_002978) Human Over-expression Lysate
Transient overexpression lysate of sodium channel, nonvoltage-gated 1, delta (SCNN1D), transcript variant 2
Storage & Handling
Cite This Product
SCNN1D (NM_002978) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006339)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SCNN1D Human Over-expression Lysates NM_002978.
Western blot validation of overexpression lysate (LS006339) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCNN1D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SCNN1D (Source: Uniprot.org, NCBI)
Amiloride-sensitive sodium channel subunit delta
amiloride-sensitive sodium channel (TC 1.A.6) family
Delta-ENaC; Delta-NaCH; DNACH; ENaCd; MGC149710; MGC149711; SCNED; sodium channel, nonvoltage-gated 1, delta; voltage-gated, type I, delta polypeptide SCNN1D ENaCd, ENaCdelta, SCNED, dNaCh sodium channel epithelial 1 subunit delta amiloride-sensitive sodium channel subunit delta|delta-ENaC|delta-NaCH|epithelial Na(+) channel subunit delta|nonvoltage-gated sodium channel 1 subunit delta|sodium channel epithelial 1 delta subunit|sodium channel, non-voltage-gated 1, delta subunit|sodium channel, nonvoltage-gated 1, delta|sodium channel, voltage-gated, type I, delta polypeptide*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006339
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