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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4)|
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Serotonin transporter (SLC6A4) (NM_001045) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4)
Storage & Handling
Cite This Product
Serotonin transporter (SLC6A4) (NM_001045) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006532)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Serotonin transporter (SLC6A4) Human Over-expression Lysates NM_001045.
Western blot validation of overexpression lysate (LS006532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC6A4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC6A4 (Source: Uniprot.org, NCBI)
Sodium-dependent serotonin transporter
sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family
5HT transporter; 5-HT Transporter; 5-HTT; 5HTTLPR; 5-HTTLPR; hSERT; HTT5-hydroxytryptamine transporter; Na+/Cl- dependent serotonin transporter; OCD1; Serotonin Transporter 1; SERT; SLC6A4; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4,5-HTTLPR; Solute carrier family 6 member 4,5HTT SLC6A4 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT solute carrier family 6 member 4 sodium-dependent serotonin transporter|5-hydroxytryptamine (serotonin) transporter|5HT transporter|Na+/Cl- dependent serotonin transporter|serotonin transporter 1|solute carrier family 6 (neurotransmitter transporter), member 4|solute carrier family 6 (neurotransmitter transporter, serotonin), member 4*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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Specific Publications For Serotonin transporter (SLC6A4) (NM_001045) Human Over-expression Lysate (LS006532)
No publications found for LS006532
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