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Product Info Summary
|Description:||Transient overexpression lysate of serpin peptidase inhibitor, clade B (ovalbumin), member 3 (SERPINB3)|
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SERPINB3 (NM_006919) Human Over-expression Lysate
Transient overexpression lysate of serpin peptidase inhibitor, clade B (ovalbumin), member 3 (SERPINB3)
Storage & Handling
Cite This Product
SERPINB3 (NM_006919) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006317)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SERPINB3 Human Over-expression Lysates NM_006919.
Western blot validation of overexpression lysate (LS006317) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SERPINB3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SERPINB3 (Source: Uniprot.org, NCBI)
HsT1196; Protein T4-A; SCC; SCCA; SCCA1; SCCA1SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; Squamous cell carcinoma antigen 1; T4-A SERPINB3 HsT1196, SCC, SCCA-1, SCCA-PD, SCCA1, SSCA1, T4-A serpin family B member 3 serpin B3|protein T4-A|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3|serpin peptidase inhibitor, clade B (ovalbumin), member 3|squamous cell carcinoma antigen 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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