SETD6 (NM_024860) Human Over-expression Lysate

Lysate of SETD6 over-expression cell lines, WB control

Transient overexpression lysate of SET domain containing 6 (SETD6), transcript variant 2

Product Info Summary

SKU: LS079918
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of SET domain containing 6 (SETD6), transcript variant 2

Product Name

SETD6 (NM_024860) Human Over-expression Lysate

See all SETD6 products

SKU/Catalog Number

LS079918

Size

100 µg

Description

Transient overexpression lysate of SET domain containing 6 (SETD6), transcript variant 2

Storage & Handling

Cite This Product

SETD6 (NM_024860) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079918)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SETD6 (Source: Uniprot.org, NCBI)

Uniprot ID

Q8TBK2

Gene ID

79918

Gene Name

SETD6

Full Name

N-lysine methyltransferase SETD6

Weight

53.189kDa

Superfamily

class V-like SAM-binding methyltransferase superfamily

Alternative Names

SET domain containing 6; SET domain-containing protein 6 SETD6 SET domain containing 6, protein lysine methyltransferase N-lysine methyltransferase SETD6|SET domain-containing protein 6

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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