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Product Info Summary
|Description:||Transient overexpression lysate of splicing factor, arginine/serine-rich 3 (SFRS3)|
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SFRS3 (SRSF3) (NM_003017) Human Over-expression Lysate
Transient overexpression lysate of splicing factor, arginine/serine-rich 3 (SFRS3)
Storage & Handling
Cite This Product
SFRS3 (SRSF3) (NM_003017) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006428)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SFRS3 (SRSF3) Human Over-expression Lysates NM_003017.
Western blot validation of overexpression lysate (LS006428) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SRSF3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SRSF3 (Source: Uniprot.org, NCBI)
Serine/arginine-rich splicing factor 3
splicing factor SR family
Pre-mRNA-splicing factor SRP20; serine/arginine-rich splicing factor 3; SFRS3splicing factor, arginine/serine-rich, 20-kD; Splicing factor, arginine/serine-rich 3SRP20; SRp20 SRSF3 SFRS3, SRp20 serine and arginine rich splicing factor 3 serine/arginine-rich splicing factor 3|epididymis secretory sperm binding protein|pre-mRNA splicing factor SRp20|pre-mRNA-splicing factor SRP20|splicing factor, arginine/serine-rich 3|splicing factor, arginine/serine-rich, 20-kD*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SRSF3, check out the SRSF3 Infographic
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No publications found for LS006428
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