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Product Info Summary
|Description:||Transient overexpression lysate of SH3-domain GRB2-like 1 (SH3GL1)|
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SH3 containing Grb 2 like 1 protein (SH3GL1) (NM_003025) Human Over-expression Lysate
Transient overexpression lysate of SH3-domain GRB2-like 1 (SH3GL1)
Storage & Handling
Cite This Product
SH3 containing Grb 2 like 1 protein (SH3GL1) (NM_003025) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006455)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SH3 containing Grb 2 like 1 protein (SH3GL1) Human Over-expression Lysates NM_003025.
Western blot validation of overexpression lysate (LS006455) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SH3GL1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SH3GL1 (Source: Uniprot.org, NCBI)
CNSA1endophilin-2; EEN fusion partner of MLL; EENSH3 domain-containing GRB2-like protein 1; Endophilin-2; extra 11-19 leukemia fusion; Extra eleven-nineteen leukemia fusion gene protein; MGC111371; SH3 domain protein 2B; SH3-containing Grb-2-like 1 protein; SH3D2Bendophilin-A2; SH3-domain GRB2-like 1; SH3P8 SH3GL1 CNSA1, EEN, SH3D2B, SH3P8 SH3 domain containing GRB2 like 1, endophilin A2 endophilin-A2|EEN fusion partner of MLL|SH3 domain protein 2B|SH3 domain-containing GRB2-like protein 1|SH3-containing Grb-2-like 1 protein|SH3-domain GRB2-like 1|endophilin-2|extra 11-19 leukemia fusion|extra eleven-nineteen leukemia fusion gene protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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Specific Publications For SH3 containing Grb 2 like 1 protein (SH3GL1) (NM_003025) Human Over-expression Lysate (LS006455)
No publications found for LS006455
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