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Product Info Summary
|Description:||Transient overexpression lysate of SH3 domain binding glutamic acid-rich protein (SH3BGR), transcript variant 1|
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SH3BGR (NM_007341) Human Over-expression Lysate
Transient overexpression lysate of SH3 domain binding glutamic acid-rich protein (SH3BGR), transcript variant 1
Storage & Handling
Cite This Product
SH3BGR (NM_007341) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006450)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SH3BGR Human Over-expression Lysates NM_007341.
Western blot validation of overexpression lysate (LS006450) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SH3BGR (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SH3BGR (Source: Uniprot.org, NCBI)
SH3 domain-binding glutamic acid-rich protein
21-GARP21-glutamic acid-rich protein; 21-Glutamic Acid-Rich Protein (21-GARP)10SH3-binding domain and glutamic acid-rich protein; SH3 domain binding glutamic acid-rich protein; SH3 domain-binding glutamic acid-rich protein; SH3BGR protein; SH3GBR SH3BGR 21-GARP SH3 domain binding glutamate rich protein SH3 domain-binding glutamic acid-rich protein|SH3-binding domain and glutamic acid-rich protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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