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Product Info Summary
|Description:||Transient overexpression lysate of SH3-binding domain protein 5-like (SH3BP5L)|
SH3BP5L (NM_030645) Human Over-expression Lysate
Transient overexpression lysate of SH3-binding domain protein 5-like (SH3BP5L)
Storage & Handling
Cite This Product
SH3BP5L (NM_030645) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080851)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SH3BP5L Human Over-expression Lysates NM_030645.
Western blot validation of overexpression lysate (LS080851) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SH3BP5L (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SH3BP5L (Source: Uniprot.org, NCBI)
SH3 domain-binding protein 5-like
FLJ33845; KIAA1720SH3BP-5-like; SH3 domain-binding protein 5-like; SH3-binding domain protein 5-like*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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