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Product Info Summary
|Description:||Transient overexpression lysate of single-minded homolog 1 (Drosophila) (SIM1)|
SIM1 (NM_005068) Human Over-expression Lysate
Transient overexpression lysate of single-minded homolog 1 (Drosophila) (SIM1)
Storage & Handling
Cite This Product
SIM1 (NM_005068) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006492)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SIM1 Human Over-expression Lysates NM_005068.
Western blot validation of overexpression lysate (LS006492) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIM1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SIM1 (Source: Uniprot.org, NCBI)
Single-minded homolog 1
Single-minded homolog 1 SIM1 bHLHe14 SIM bHLH transcription factor 1 single-minded homolog 1|class E basic helix-loop-helix protein 14|single-minded family bHLH transcription factor 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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