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Product Info Summary
|Description:||Transient overexpression lysate of single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s|
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SIM2 (NM_009586) Human Over-expression Lysate
Transient overexpression lysate of single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s
Storage & Handling
Cite This Product
SIM2 (NM_009586) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006493)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SIM2 Human Over-expression Lysates NM_009586.
Western blot validation of overexpression lysate (LS006493) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIM2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SIM2 (Source: Uniprot.org, NCBI)
Single-minded homolog 2
human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15; short isoform; single-minded (Drosophila) homolog 2; single-minded homolog 2 (Drosophila); transcription factor SIM2 SIM2 HMC13F06, HMC29C01, SIM, bHLHe15 SIM bHLH transcription factor 2 single-minded homolog 2|class E basic helix-loop-helix protein 15|single-minded family bHLH transcription factor 2|transcription factor SIM2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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