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Product Info Summary
|Description:||Transient overexpression lysate of polymerase (DNA-directed), delta interacting protein 3 (POLDIP3), transcript variant 1|
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SKAR (POLDIP3) (NM_032311) Human Over-expression Lysate
Transient overexpression lysate of polymerase (DNA-directed), delta interacting protein 3 (POLDIP3), transcript variant 1
Storage & Handling
Cite This Product
SKAR (POLDIP3) (NM_032311) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084271)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SKAR (POLDIP3) Human Over-expression Lysates NM_032311.
Western blot validation of overexpression lysate (LS084271) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLDIP3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for POLDIP3 (Source: Uniprot.org, NCBI)
Polymerase delta-interacting protein 3
KIAA1649polymerase delta interacting protein 46; p46; PDIP46S6K1 Aly/REF-like target; polymerase (DNA-directed), delta interacting protein 3; RNA-binding protein P46; SKARpolymerase delta-interacting protein 3,46 kDa DNA polymerase delta interaction protein POLDIP3 PDIP3, PDIP46, SKAR DNA polymerase delta interacting protein 3 polymerase delta-interacting protein 3|46 kDa DNA polymerase delta interaction protein|RNA-binding protein P46|S6K1 Aly/REF-like target|polymerase (DNA) delta interacting protein 3|polymerase (DNA-directed), delta interacting protein 3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on POLDIP3, check out the POLDIP3 Infographic
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In this infographic, you will see the following information for POLDIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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