SKAR (POLDIP3) (NM_032311) Human Over-expression Lysate

Lysate of SKAR over-expression cell lines, WB control

Transient overexpression lysate of polymerase (DNA-directed), delta interacting protein 3 (POLDIP3), transcript variant 1

Product Info Summary

SKU: LS084271
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of polymerase (DNA-directed), delta interacting protein 3 (POLDIP3), transcript variant 1

Product Name

SKAR (POLDIP3) (NM_032311) Human Over-expression Lysate

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SKU/Catalog Number

LS084271

Size

100 µg

Description

Transient overexpression lysate of polymerase (DNA-directed), delta interacting protein 3 (POLDIP3), transcript variant 1

Storage & Handling

Cite This Product

SKAR (POLDIP3) (NM_032311) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084271)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for POLDIP3 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9BY77

Gene ID

84271

Gene Name

POLDIP3

Full Name

Polymerase delta-interacting protein 3

Weight

46.089kDa

Alternative Names

KIAA1649polymerase delta interacting protein 46; p46; PDIP46S6K1 Aly/REF-like target; polymerase (DNA-directed), delta interacting protein 3; RNA-binding protein P46; SKARpolymerase delta-interacting protein 3,46 kDa DNA polymerase delta interaction protein POLDIP3 PDIP3, PDIP46, SKAR DNA polymerase delta interacting protein 3 polymerase delta-interacting protein 3|46 kDa DNA polymerase delta interaction protein|RNA-binding protein P46|S6K1 Aly/REF-like target|polymerase (DNA) delta interacting protein 3|polymerase (DNA-directed), delta interacting protein 3

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on POLDIP3, check out the POLDIP3 Infographic

POLDIP3 infographic

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In this infographic, you will see the following information for POLDIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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