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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)|
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SLC10A1 (NM_003049) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)
Storage & Handling
Cite This Product
SLC10A1 (NM_003049) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006554)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC10A1 Human Over-expression Lysates NM_003049.
Western blot validation of overexpression lysate (LS006554) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC10A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC10A1 (Source: Uniprot.org, NCBI)
Sodium/bile acid cotransporter
bile acid:sodium symporter (BASS) (TC 2.A.28) family
Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Na/taurocholate cotransporting polypeptide; NTCP; NTCP1; NTCPgrowth-inhibiting protein 29; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; Sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; Solute carrier family 10 member 1 SLC10A1 NTCP solute carrier family 10 member 1 sodium/bile acid cotransporter|Na(+)/bile acid cotransporter|Na(+)/taurocholate transport protein|Na/taurocholate cotransporting polypeptide|cell growth-inhibiting gene 29 protein|growth-inhibiting protein 29|sodium/taurocholate cotransporter|sodium/taurocholate cotransporting polypeptide|solute carrier family 10 (sodium/bile acid cotransporter family), member 1|solute carrier family 10 (sodium/bile acid cotransporter), member 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006554
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