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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 19 (folate transporter), member 1 (SLC19A1)|
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SLC19A1 (NM_194255) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 19 (folate transporter), member 1 (SLC19A1)
Storage & Handling
Cite This Product
SLC19A1 (NM_194255) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006573)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC19A1 Human Over-expression Lysates NM_194255.
Western blot validation of overexpression lysate (LS006573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC19A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC19A1 (Source: Uniprot.org, NCBI)
Reduced folate transporter
reduced folate carrier (RFC) transporter (TC 2.A.48) family
CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1 SLC19A1 CHMD, FOLT, IFC-1, IFC1, REFC, RFC, RFC1, RFT-1, hRFC, hSLC19A1 solute carrier family 19 member 1 reduced folate transporter|folate transporter 1|intestinal folate carrier 1|placental folate transporter|reduced folate carrier 1|reduced folate carrier protein|reduced folate transporter 1|solute carrier family 19 (folate transporter), member 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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