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Product Info Summary
|Description:||Transient overexpression lysate of major facilitator superfamily domain containing 7 (MFSD7)|
SLC49A3 (NM_032219) Human Over-expression Lysate
Transient overexpression lysate of major facilitator superfamily domain containing 7 (MFSD7)
Storage & Handling
Cite This Product
SLC49A3 (NM_032219) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084179)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC49A3 Human Over-expression Lysates NM_032219.
Western blot validation of overexpression lysate (LS084179) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MFSD7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC49A3 (Source: Uniprot.org, NCBI)
Solute carrier family 49 member A3
major facilitator superfamily
Solute carrier family 49 member A3 SLC49A3 LP2561, MFSD7 solute carrier family 49 member 3 solute carrier family 49 member A3|MYL5|major facilitator superfamily domain containing 7|major facilitator superfamily domain-containing protein 7|myosin light polypeptide 5 regulatory protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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