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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)|
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SLC9A1 (NM_003047) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)
Storage & Handling
Cite This Product
SLC9A1 (NM_003047) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006548)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC9A1 Human Over-expression Lysates NM_003047.
Western blot validation of overexpression lysate (LS006548) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC9A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC9A1 (Source: Uniprot.org, NCBI)
Sodium/hydrogen exchanger 1
monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
APNH; APNH1; APNHFLJ42224; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na+/H+, amiloride sensitive); Na-Li countertransporter; NHE1; NHE-1; NHE1Na+/H+ antiporter, amiloride-sensitive; SLC9A1; sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1; Solute carrier family 9 member 1 SLC9A1 APNH, LIKNS, NHE-1, NHE1, PPP1R143 solute carrier family 9 member A1 sodium/hydrogen exchanger 1|Na(+)/H(+) exchanger 1|Na-Li countertransporter|protein phosphatase 1, regulatory subunit 143|solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive)|solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006548
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