SLC9A1 (NM_003047) Human Over-expression Lysate

Lysate of NHE1/SLC9A1 over-expression cell lines, WB control

Transient overexpression lysate of solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)

Product Info Summary

SKU: LS006548
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)

Product Name

SLC9A1 (NM_003047) Human Over-expression Lysate

See all NHE1/SLC9A1 products

SKU/Catalog Number

LS006548

Size

100 µg

Description

Transient overexpression lysate of solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)

Storage & Handling

Cite This Product

SLC9A1 (NM_003047) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006548)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SLC9A1 (Source: Uniprot.org, NCBI)

Gene Name

SLC9A1

Full Name

Sodium/hydrogen exchanger 1

Weight

90.763kDa

Superfamily

monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Alternative Names

APNH; APNH1; APNHFLJ42224; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na+/H+, amiloride sensitive); Na-Li countertransporter; NHE1; NHE-1; NHE1Na+/H+ antiporter, amiloride-sensitive; SLC9A1; sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1; Solute carrier family 9 member 1 SLC9A1 APNH, LIKNS, NHE-1, NHE1, PPP1R143 solute carrier family 9 member A1 sodium/hydrogen exchanger 1|Na(+)/H(+) exchanger 1|Na-Li countertransporter|protein phosphatase 1, regulatory subunit 143|solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive)|solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SLC9A1, check out the SLC9A1 Infographic

SLC9A1 infographic

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In this infographic, you will see the following information for SLC9A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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