SMARCD2 (NM_001098426) Human Over-expression Lysate

Lysate of SMARCD2 over-expression cell lines, WB control

Transient overexpression lysate of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2), transcript variant 1

Product Info Summary

SKU: LS006603
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2), transcript variant 1

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Product Name

SMARCD2 (NM_001098426) Human Over-expression Lysate

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SKU/Catalog Number

LS006603

Size

100 µg

Description

Transient overexpression lysate of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2), transcript variant 1

Storage & Handling

Cite This Product

SMARCD2 (NM_001098426) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006603)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SMARCD2 (Source: Uniprot.org, NCBI)

Gene Name

SMARCD2

Full Name

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2

Weight

58.921kDa

Superfamily

SMARCD family

Alternative Names

60 kDa BRG-1/Brm-associated factor subunit B; BAF60B; BRG1-associated factor 60B; chromatin remodeling complex BAF60B subunit; CRACD2; mammalian chromatin remodeling complex BRG1-associated factor 60B; PRO2451; Rsc6p; SWI/SNF complex 60 kDa subunit B; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2; Swp73-like protein SMARCD2 BAF60B, CRACD2, PRO2451, Rsc6p, SGD2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2|60 kDa BRG-1/Brm-associated factor subunit B|BRG1-associated factor 60B|SWI/SNF complex 60 kDa subunit B|Swp73-like protein|chromatin remodeling complex BAF60B subunit|mammalian chromatin remodeling complex BRG1-associated factor 60B

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SMARCD2, check out the SMARCD2 Infographic

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