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Product Info Summary
|Description:||Transient overexpression lysate of small nuclear RNA activating complex, polypeptide 2, 45kDa (SNAPC2), transcript variant 1|
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SNAP45 (SNAPC2) (NM_003083) Human Over-expression Lysate
Transient overexpression lysate of small nuclear RNA activating complex, polypeptide 2, 45kDa (SNAPC2), transcript variant 1
Storage & Handling
Cite This Product
SNAP45 (SNAPC2) (NM_003083) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006618)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SNAP45 (SNAPC2) Human Over-expression Lysates NM_003083.
Western blot validation of overexpression lysate (LS006618) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SNAPC2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SNAPC2 (Source: Uniprot.org, NCBI)
snRNA-activating protein complex subunit 2
Proximal sequence element-binding transcription factor subunit delta; PSE-binding factor subunit delta; PTF subunit delta; PTFdelta; small nuclear RNA activating complex, polypeptide 2, 45kD; small nuclear RNA activating complex, polypeptide 2, 45kDa; Small nuclear RNA-activating complex polypeptide 2; SNAP45snRNA-activating protein complex 45 kDa subunit; SNAPc 45 kDa subunit; SNAPc subunit 2; snRNA-activating protein complex subunit 2 SNAPC2 PTFDELTA, SNAP45 small nuclear RNA activating complex polypeptide 2 snRNA-activating protein complex subunit 2|PSE-binding factor subunit delta|PTF subunit delta|SNAPC 45 kDa subunit|SNAPc subunit 2|proximal sequence element-binding transcription factor subunit delta|small nuclear RNA activating complex, polypeptide 2, 45kD|small nuclear RNA activating complex, polypeptide 2, 45kDa|snRNA-activating protein complex 45 kDa subunit*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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