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Product Info Summary
|Description:||Transient overexpression lysate of small nuclear RNA activating complex, polypeptide 3, 50kDa (SNAPC3)|
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SNAPC3 (NM_001039697) Human Over-expression Lysate
Transient overexpression lysate of small nuclear RNA activating complex, polypeptide 3, 50kDa (SNAPC3)
Storage & Handling
Cite This Product
SNAPC3 (NM_001039697) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006619)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SNAPC3 Human Over-expression Lysates NM_001039697.
Western blot validation of overexpression lysate (LS006619) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SNAPC3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SNAPC3 (Source: Uniprot.org, NCBI)
snRNA-activating protein complex subunit 3
MGC132011; MGC33124; Proximal sequence element-binding transcription factor subunit beta; PSE-binding factor subunit beta; PTF subunit beta; PTFbeta; small nuclear RNA activating complex, polypeptide 3, 50kD; small nuclear RNA activating complex, polypeptide 3, 50kDa; Small nuclear RNA-activating complex polypeptide 3; SNAP50SNAPc 50 kDa subunit; SNAPc subunit 3; snRNA-activating protein complex 50 kDa subunit; snRNA-activating protein complex subunit 3 SNAPC3 PTFbeta, SNAP50 small nuclear RNA activating complex polypeptide 3 snRNA-activating protein complex subunit 3|PSE-binding factor subunit beta|PTF subunit beta|SNAPc 50 kDa subunit|SNAPc subunit 3|proximal sequence element-binding transcription factor subunit beta|small nuclear RNA activating complex, polypeptide 3, 50kD|small nuclear RNA activating complex, polypeptide 3, 50kDa|snRNA-activating protein complex 50 kDa subunit*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006619
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