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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 22 (organic cation/carnitine transporter), member 5 (SLC22A5)|
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Solute carrier family 22 member 5 (SLC22A5) (NM_003060) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 22 (organic cation/carnitine transporter), member 5 (SLC22A5)
Storage & Handling
Cite This Product
Solute carrier family 22 member 5 (SLC22A5) (NM_003060) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006584)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Solute carrier family 22 member 5 (SLC22A5) Human Over-expression Lysates NM_003060.
Western blot validation of overexpression lysate (LS006584) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC22A5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC22A5 (Source: Uniprot.org, NCBI)
Solute carrier family 22 member 5
major facilitator (TC 2.A.1) superfamily
CDSP; FLJ46769; High-affinity sodium-dependent carnitine cotransporter; OCTN2; OCTN2high-affinity sodium dependent carnitine cotransporter; OCTN2VT; organic cation transporter 2; organic cation transporter 5; Organic cation/carnitine transporter 2; SCD; SLC22A5; solute carrier family 22 (organic cation transporter), member 5; solute carrier family 22 (organic cation/carnitine transporter), member 5; solute carrier family 22 member 5 SLC22A5 CDSP, OCTN2 solute carrier family 22 member 5 solute carrier family 22 member 5|high-affinity sodium dependent carnitine cotransporter|organic cation/carnitine transporter 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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Specific Publications For Solute carrier family 22 member 5 (SLC22A5) (NM_003060) Human Over-expression Lysate (LS006584)
No publications found for LS006584
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