SPAG4 (NM_003116) Human Over-expression Lysate

Lysate of SPAG4 over-expression cell lines, WB control

Transient overexpression lysate of sperm associated antigen 4 (SPAG4)

Product Info Summary

SKU: LS006676
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of sperm associated antigen 4 (SPAG4)

Product Name

SPAG4 (NM_003116) Human Over-expression Lysate

See all SPAG4 products

SKU/Catalog Number

LS006676

Size

100 µg

Description

Transient overexpression lysate of sperm associated antigen 4 (SPAG4)

Storage & Handling

Cite This Product

SPAG4 (NM_003116) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006676)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SPAG4 (Source: Uniprot.org, NCBI)

Gene Name

SPAG4

Full Name

Sperm-associated antigen 4 protein

Weight

48.165kDa

Alternative Names

acrosomal protein ACR55; CT127; Outer dense fiber-associated protein SPAG4; Sad1 and UNC84 domain containing 4; sperm associated antigen 4; sperm tail protein; sperm-associated antigen 4 protein; SUN domain-containing protein 4; SUN4cancer/testis antigen 127 SPAG4 CT127, SUN4 sperm associated antigen 4 sperm-associated antigen 4 protein|SUN domain-containing protein 4|Sad1 and UNC84 domain containing 4|acrosomal protein ACR55|cancer/testis antigen 127|outer dense fiber-associated protein SPAG4|sperm tail protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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