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Product Info Summary
| SKU: | LS006687 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1 |
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Product info
Product Name
SPG7 (NM_003119) Human Over-expression Lysate
SKU/Catalog Number
LS006687
Size
100 µg
Description
Transient overexpression lysate of spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1
Storage & Handling
Cite This Product
SPG7 (NM_003119) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006687)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SPG7 Human Over-expression Lysates NM_003119.
Western blot validation of overexpression lysate (LS006687) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SPG7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SPG7 (Source: Uniprot.org, NCBI)
Gene Name
SPG7
Full Name
Paraplegin
Weight
88.235kDa
Alternative Names
CARMGC126331; cell matrix adhesion regulator; CMARMGC126332; EC 3.4.24; EC 3.4.24.-; FLJ37308; paraplegin; PGNcell adhesion regulator; spastic paraplegia 7 (pure and complicated autosomal recessive); Spastic paraplegia 7 protein; SPG5C SPG7 CAR, CMAR, PGN, SPG5C SPG7 matrix AAA peptidase subunit, paraplegin paraplegin|SPG7, paraplegin matrix AAA peptidase subunit|cell matrix adhesion regulator|spastic paraplegia 7 (pure and complicated autosomal recessive)|spastic paraplegia 7 protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SPG7, check out the SPG7 Infographic
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In this infographic, you will see the following information for SPG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SPG7 (NM_003119) Human Over-expression Lysate (LS006687)
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No publications found for LS006687
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