SPHKAP (NM_030623) Human Over-expression Lysate

Lysate of SPHKAP over-expression cell lines, WB control

Transient overexpression lysate of SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2

Product Info Summary

SKU: LS080309
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2

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Product Name

SPHKAP (NM_030623) Human Over-expression Lysate

See all SPHKAP products

SKU/Catalog Number

LS080309

Size

100 µg

Description

Transient overexpression lysate of SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2

Storage & Handling

Cite This Product

SPHKAP (NM_030623) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080309)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SPHKAP (Source: Uniprot.org, NCBI)

Uniprot ID

Q2M3C7

Gene ID

80309

Gene Name

SPHKAP

Full Name

A-kinase anchor protein SPHKAP

Weight

186.456kDa

Superfamily

AKAP110 family

Alternative Names

A-kinase anchor protein SPHKAP; SKIP; sphingosine kinase type 1-interacting protein; SPHK1 (sphingosine kinase type 1) interacting protein; SPHK1 interactor, AKAP domain containing; SPHK1-interactor and AKAP domain-containing protein SPHKAP SKIP SPHK1 interactor, AKAP domain containing A-kinase anchor protein SPHKAP|SPHK1 (sphingosine kinase type 1) interacting protein|SPHK1-interactor and AKAP domain-containing protein|sphingosine kinase type 1-interacting protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SPHKAP, check out the SPHKAP Infographic

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