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Product Info Summary
|Description:||Transient overexpression lysate of splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1)|
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SPSB1 (NM_025106) Human Over-expression Lysate
Transient overexpression lysate of splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1)
Storage & Handling
Cite This Product
SPSB1 (NM_025106) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080176)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SPSB1 Human Over-expression Lysates NM_025106.
Western blot validation of overexpression lysate (LS080176) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SPSB1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SPSB1 (Source: Uniprot.org, NCBI)
SPRY domain-containing SOCS box protein 1
novel SPRY domain containing protein; splA/ryanodine receptor domain and SOCS box containing 1; SPRY domain-containing SOCS box protein 1; SSB-1SPRY domain-containing SOCS box protein SSB-1; SSB1SPSB Spsb1|1110014L01Rik, 4930422J18Rik, AI596360, AI854583, SS, SSB-1, SSB1|splA/ryanodine receptor domain and SOCS box containing 1|SPRY domain-containing SOCS box protein 1|SPRY domain-containing SOCS box protein SSB-1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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