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Product Info Summary
|Description:||Transient overexpression lysate of structure specific recognition protein 1 (SSRP1)|
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SSRP1 (NM_003146) Human Over-expression Lysate
Transient overexpression lysate of structure specific recognition protein 1 (SSRP1)
Storage & Handling
Cite This Product
SSRP1 (NM_003146) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006749)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SSRP1 Human Over-expression Lysates NM_003146.
Western blot validation of overexpression lysate (LS006749) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SSRP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SSRP1 (Source: Uniprot.org, NCBI)
FACT complex subunit SSRP1
Chromatin-specific transcription elongation factor 80 kDa subunit; cisplatin-DNA SSRP; facilitates chromatin remodeling 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; Facilitates chromatin transcription complex subunit SSRP1; FACT complex subunit SSRP1; FACT; FACT80FACT 80 kDa subunit; FACTp80; high mobility group box; hSSRP1; Recombination signal sequence recognition protein 1; structure specific recognition protein 1; Structure-specific recognition protein 1; T160 SSRP1 FACT, FACT80, T160 structure specific recognition protein 1 FACT complex subunit SSRP1|FACT 80 kDa subunit|FACTp80|chromatin-specific transcription elongation factor 80 kDa subunit|cisplatin-DNA SSRP|facilitates chromatin remodeling 80 kDa subunit|facilitates chromatin transcription complex 80 kDa subunit|facilitates chromatin transcription complex subunit SSRP1|hSSRP1|high mobility group box|recombination signal sequence recognition protein 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SSRP1, check out the SSRP1 Infographic
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