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Product Info Summary
|Description:||Transient overexpression lysate of ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 (ST6GALNAC5)|
ST6GALNAC5 (NM_030965) Human Over-expression Lysate
Transient overexpression lysate of ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 (ST6GALNAC5)
Storage & Handling
Cite This Product
ST6GALNAC5 (NM_030965) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081849)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ST6GALNAC5 Human Over-expression Lysates NM_030965.
Western blot validation of overexpression lysate (LS081849) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ST6GALNAC5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ST6GALNAC5 (Source: Uniprot.org, NCBI)
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5
glycosyltransferase 29 family
alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase E; EC 2.4.99; EC 2.4.99.-; EC 18.104.22.168; GalNAc alpha-2,6-sialyltransferase V; GD1 alpha synthase; MGC3184; sialyltransferase 7 (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase) E; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) E; Sialyltransferase 7E; SIAT7E; SIAT7-E; SIAT7Ealpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V; ST6 (alpha-N-acetyl-neuraminyl-2 ST6GALNAC5 SIAT7-E, SIAT7E, ST6GalNAcV ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5|GD1 alpha synthase|GalNAc alpha-2,6-sialyltransferase V|ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5|ST6 GalNAc alpha-2,6-sialyltransferase 5|ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5|ST6GalNAc V|alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V|alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase E|sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) E|sialyltransferase 7E*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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