STK16 (NM_001008910) Human Over-expression Lysate

Lysate of STK16 over-expression cell lines, WB control

Transient overexpression lysate of serine/threonine kinase 16 (STK16), transcript variant 1

Product Info Summary

SKU: LS008576
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of serine/threonine kinase 16 (STK16), transcript variant 1

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Product Name

STK16 (NM_001008910) Human Over-expression Lysate

See all STK16 products

SKU/Catalog Number

LS008576

Size

100 µg

Description

Transient overexpression lysate of serine/threonine kinase 16 (STK16), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

STK16 (NM_001008910) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008576)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for STK16 (Source: Uniprot.org, NCBI)

Gene Name

STK16

Full Name

Serine/threonine-protein kinase 16

Weight

34.656kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11.1; FLJ39635; hPSK; MGC16211; MPSK1; MPSKFLJ39635; Myristoylated and palmitoylated serine/threonine-protein kinase; PKL12KRCT; protein kinase expressed in day 12 fetal liver; Protein kinase PKL12; serine/threonine kinase 16; serine/threonine-protein kinase 16; TGF-beta-stimulated factor 1; TSF1; TSF-1 STK16 KRCT, MPSK, PKL12, PSK, TSF1, hPSK serine/threonine kinase 16 serine/threonine-protein kinase 16|TGF-beta-stimulated factor 1|myristoylated and palmitoylated serine/threonine-protein kinase|protein kinase PKL12|protein kinase expressed in day 12 fetal liver|tyrosine-protein kinase STK16

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on STK16, check out the STK16 Infographic

STK16 infographic

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In this infographic, you will see the following information for STK16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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