Product Info Summary
SKU: | LS006647 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of superoxide dismutase 1, soluble (SOD1) |
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Product info
Product Name
Superoxide Dismutase 1 (SOD1) (NM_000454) Human Over-expression Lysate
See all SOD1/Cu-Zn SOD products
SKU/Catalog Number
LS006647
Size
100 µg
Description
Transient overexpression lysate of superoxide dismutase 1, soluble (SOD1)
Storage & Handling
Cite This Product
Superoxide Dismutase 1 (SOD1) (NM_000454) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006647)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Superoxide Dismutase 1 (SOD1) Human Over-expression Lysates NM_000454.
Western blot validation of overexpression lysate (LS006647) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SOD1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SOD1 (Source: Uniprot.org, NCBI)
Gene Name
SOD1
Full Name
Superoxide dismutase [Cu-Zn]
Weight
15.936kDa
Superfamily
Cu-Zn superoxide dismutase family
Alternative Names
ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic SOD1 ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer superoxide dismutase 1 superoxide dismutase [Cu-Zn]|Cu/Zn superoxide dismutase|SOD, soluble|epididymis secretory protein Li 44|indophenoloxidase A|superoxide dismutase 1, soluble|superoxide dismutase, cystolic
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SOD1, check out the SOD1 Infographic
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In this infographic, you will see the following information for SOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Superoxide Dismutase 1 (SOD1) (NM_000454) Human Over-expression Lysate (LS006647)
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