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Product Info Summary
| SKU: | PROTP42831 |
|---|---|
| Size: | 5ug,20ug |
| Origin Species: | Swine |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Swine recombinant CCL2 (C-C Motif Chemokine Ligand 2) protein, AF
View all CCL2 recombinant proteins
SKU/Catalog Number
PROTP42831
Size
5ug,20ug
Tag
His Tag (N-term)
Description
CCL2 (Chemokine ligand 2) is also named for the MCP1 (monocyte chemoattractant protein 1) which is a cytokine of CC chemokine family. CCL2 plays an important role in the recruitment of T cell and monocyte during inflammation, which is induced by the infection. CCL2 is secreted from the osteoblasts which is under the mediation of the NF-κB pathway. In cancer therapy, CCL2 plays an important role in increasing the anti-tumor capability of monocytes and neutrophils.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Swine recombinant CCL2 (C-C Motif Chemokine Ligand 2) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP42831)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Molecular weight
The protein has a calculated MW of 9.42 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
QPDAINSPVTCCYTLTSKKISMQRLMSYRRVTSSKCPKEAVIFKTIAGKEICAEPKQKWVQDSISHLDKKNQTPKP with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant swine CCL2
Specific Publications For Swine recombinant CCL2 (C-C Motif Chemokine Ligand 2) protein, AF (PROTP42831)
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