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Product Info Summary
|Description:||Transient overexpression lysate of syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) (SNTA1)|
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Syntrophin alpha 1 (SNTA1) (NM_003098) Human Over-expression Lysate
Transient overexpression lysate of syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) (SNTA1)
Storage & Handling
Cite This Product
Syntrophin alpha 1 (SNTA1) (NM_003098) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006640)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Syntrophin alpha 1 (SNTA1) Human Over-expression Lysates NM_003098.
Western blot validation of overexpression lysate (LS006640) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SNTA1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SNTA1 (Source: Uniprot.org, NCBI)
59 kDa dystrophin-associated protein A1 acidic component 1; dJ1187J4.5; LQT12; Pro-TGF-alpha cytoplasmic domain-interacting protein 1; SNT1alpha-1-syntrophin; syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component); syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component); syntrophin-1; TACIP1acidic alpha 1 syntrophin SNTA1 LQT12, SNT1, TACIP1, dJ1187J4.5 syntrophin alpha 1 alpha-1-syntrophin|59 kDa dystrophin-associated protein A1 acidic component 1|acidic alpha 1 syntrophin|dystrophin-associated protein A1, 59kDa, acidic component|pro-TGF-alpha cytoplasmic domain-interacting protein 1|syntrophin-1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SNTA1, check out the SNTA1 Infographic
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Specific Publications For Syntrophin alpha 1 (SNTA1) (NM_003098) Human Over-expression Lysate (LS006640)
No publications found for LS006640
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