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Product Info Summary
|Description:||Transient overexpression lysate of TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11)|
TAF11 (NM_005643) Human Over-expression Lysate
Transient overexpression lysate of TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11)
Storage & Handling
Cite This Product
TAF11 (NM_005643) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006882)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TAF11 Human Over-expression Lysates NM_005643.
Western blot validation of overexpression lysate (LS006882) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TAF11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TAF11 (Source: Uniprot.org, NCBI)
Transcription initiation factor TFIID subunit 11
28kDa; TAF(II)28; TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2IMGC:15243; TAFII-28; TAFII28TFIID subunit p30-beta; TATA box binding protein (TBP)-associated factor, RNA polymerase II, I, 28kD; transcription initiation factor TFIID 28 kD subunit; Transcription initiation factor TFIID 28 kDa subunit; transcription initiation factor TFIID subunit 11 TAF11 MGC:15243, PRO2134, TAF2I, TAFII28 TATA-box binding protein associated factor 11 transcription initiation factor TFIID subunit 11|TAF(II)28|TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa|TAFII-28|TATA box binding protein (TBP)-associated factor, RNA polymerase II, I, 28kD|TFIID subunit p30-beta|transcription initiation factor TFIID 28 kD subunit|transcription initiation factor TFIID 28 kDa subunit*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006882
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