TAL1 (NM_003189) Human Over-expression Lysate

Lysate of SCL/Tal1 over-expression cell lines, WB control

Transient overexpression lysate of T-cell acute lymphocytic leukemia 1 (TAL1)

Product Info Summary

SKU: LS006886
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of T-cell acute lymphocytic leukemia 1 (TAL1)

Product Name

TAL1 (NM_003189) Human Over-expression Lysate

See all SCL/Tal1 products

SKU/Catalog Number

LS006886

Size

100 µg

Description

Transient overexpression lysate of T-cell acute lymphocytic leukemia 1 (TAL1)

Storage & Handling

Cite This Product

TAL1 (NM_003189) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006886)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for TAL1 (Source: Uniprot.org, NCBI)

Gene Name

TAL1

Full Name

T-cell acute lymphocytic leukemia protein 1

Weight

34.271kDa

Alternative Names

BHLHA17; bHLHa17tal-1; Class A basic helix-loop-helix protein 17; SCL; SCLT-cell acute lymphocytic leukemia protein 1; Stem Cell Protein; Tal1; TAL-1; T-cell acute lymphocytic leukemia 1; T-cell leukemia/lymphoma protein 5; TCL5; TCL5tal-1 product TAL1 SCL, TCL5, bHLHa17, tal-1 TAL bHLH transcription factor 1, erythroid differentiation factor T-cell acute lymphocytic leukemia protein 1|T-cell acute lymphocytic leukemia 1|T-cell leukemia/lymphoma protein 5|class A basic helix-loop-helix protein 17|stem cell protein|tal-1 product

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on TAL1, check out the TAL1 Infographic

TAL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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