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Product Info Summary
|Description:||Transient overexpression lysate of T-cell acute lymphocytic leukemia 1 (TAL1)|
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TAL1 (NM_003189) Human Over-expression Lysate
Transient overexpression lysate of T-cell acute lymphocytic leukemia 1 (TAL1)
Storage & Handling
Cite This Product
TAL1 (NM_003189) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006886)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TAL1 Human Over-expression Lysates NM_003189.
Western blot validation of overexpression lysate (LS006886) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TAL1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TAL1 (Source: Uniprot.org, NCBI)
T-cell acute lymphocytic leukemia protein 1
BHLHA17; bHLHa17tal-1; Class A basic helix-loop-helix protein 17; SCL; SCLT-cell acute lymphocytic leukemia protein 1; Stem Cell Protein; Tal1; TAL-1; T-cell acute lymphocytic leukemia 1; T-cell leukemia/lymphoma protein 5; TCL5; TCL5tal-1 product TAL1 SCL, TCL5, bHLHa17, tal-1 TAL bHLH transcription factor 1, erythroid differentiation factor T-cell acute lymphocytic leukemia protein 1|T-cell acute lymphocytic leukemia 1|T-cell leukemia/lymphoma protein 5|class A basic helix-loop-helix protein 17|stem cell protein|tal-1 product*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on TAL1, check out the TAL1 Infographic
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No publications found for LS006886
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