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Product Info Summary
|Description:||Transient overexpression lysate of transmembrane protein 120A (TMEM120A)|
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TMEM120A (NM_031925) Human Over-expression Lysate
Transient overexpression lysate of transmembrane protein 120A (TMEM120A)
Storage & Handling
Cite This Product
TMEM120A (NM_031925) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083862)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TMEM120A Human Over-expression Lysates NM_031925.
Western blot validation of overexpression lysate (LS083862) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TMEM120A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TMEM120A (Source: Uniprot.org, NCBI)
Transmembrane protein 120A
TMPITNET29; transmembrane protein 120A; Transmembrane protein induced by tumor necrosis factor alpha TMEM120A NET29, TMPIT transmembrane protein 120A ion channel TACAN|transmembrane protein induced by tumor necrosis factor alpha*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS083862
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