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Product Info Summary
| SKU: | EKC1672 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
TNR16 Colorimetric Cell-Based ELISA
See all NGFR/TNFRSF16/p75NTR products
SKU/Catalog Number
EKC1672
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TNR16 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TNR16 protein expression profile in cells. The kit can be used for measuring the relative amounts of TNR16 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TNR16.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TNR16 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1672)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1672 is reactive to NGFR in Human, Mouse, Rat samples
Application Guarantee
EKC1672 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-NGFR Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HepG2 cells
Protein Target Info & Infographic
Gene/Protein Information For NGFR (Source: Uniprot.Org, NCBI)
Gene Name
NGFR
Full Name
Tumor necrosis factor receptor superfamily member 16
Weight
45.183kDa
Alternative Names
CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16 NGFR CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR nerve growth factor receptor tumor necrosis factor receptor superfamily member 16|NGF receptor|TNFR superfamily, member 16|low affinity neurotrophin receptor p75NTR|low-affinity nerve growth factor receptor|p75 ICD
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NGFR, check out the NGFR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NGFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TNR16 Colorimetric Cell-Based ELISA (EKC1672)
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for TNR16 Colorimetric Cell-Based ELISA
Question
Does EKC1672 kit have a special plate coated with antibodies? If it is an uncoated culture plate, Is it possible to perform assays on similar plates?
Verified customer
Asked: 2020-01-10
Answer
The plate included in the TNR16 Colorimetric Cell-Based ELISA EKC1672 kit is not coated with antibodies, therefore, you can perform the assays on similar plates just as long as the plates are for cell culture.
Boster Scientific Support
Answered: 2020-01-10
Question
Regarding step 11 in the assay protocol for EKC1672 kit, If you add NGFR and GAPDH antibody to the same well, how do you adjust at GAPDH? Should we separate wells for NGFR and GAPDH?
Verified customer
Asked: 2019-01-14
Answer
For the TNR16 Colorimetric Cell-Based ELISA EKC1672 kit, GAPDH is a housekeeping gene and is used to normalize. So you don't need to adjust GAPDH. Also, no need to separate the wells for NGFR and GAPDH.
Boster Scientific Support
Answered: 2019-01-14
