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- Table of Contents
NGFR antibody (A01187) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of A431 cells using anti-p75 NGF Receptor/NGFR antibody (A01187).
Overlay histogram showing A431 cells stained with A01187 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p75 NGF Receptor/NGFR Antibody (A01187, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A01187 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p75 NGF Receptor/NGFR Antibody (A01187, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-p75 NGF Receptor/NGFR Antibody Picoband®
NGFR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
NGFR antibody was positively detected by WB in:
rat brain tissue, human Sw620 whole cell, human Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NGFR antibody was positively detected by ICC/IF in:
A431 cell
NGFR antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Rat
Tested
Recommend
Human
Tested
Recommend
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Mouse
Predicted
Predicted
Product Information
Product Name
Anti-p75 NGF Receptor/NGFR Antibody Picoband®
View all NGFR Antibodies
View all NGFR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human p75 NGF Receptor/NGFR recombinant protein (Position: K29-Q240).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa, 75 kDa
Calculated Molecular Weight
45.2 kDa
Background of NGFR
The low-affinity nerve growth factor receptor (nerve growth factor receptor (TNFR superfamily, member 16), also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. LNGFR is a member of the tumor necrosis factor receptor (TNF receptor)superfamily indeed, LNGFR was the first member of this large family of receptors to be characterized. It is mapped to 17q21.33. Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
Cite This Product
Anti-p75 NGF Receptor/NGFR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01187)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Dog
95%
High Homology
Rabbit
93%
High Homology
Rat
93%
High Homology
Mouse
92%
High Homology
Zebrafish
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NGFR antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NGFR antibody
Flow Cytometry (FC) protocol for NGFR antibody
Publications
Application:
Species:
Transplantation of olfactory ensheathing cells can alleviate neuroinflammatory responses in rats with trigeminal neuralgia
Published: 2023-12-15
Journal: BRAIN RESEARCH
Application: ICC
Impact Factor: 2.90
Species: Rat
PMID: 38104922
In vitro biocompatibility study of EDC/NHS cross-linked silk fibroin scaffold with olfactory ensheathing cells
Published: 2022-11-10
Journal: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Application: ICC
Impact Factor: 3.68
Species: Rat
PMID: 36285432
Synthesis of Double Interfering Biodegradable Nano-MgO Micelle Composites and Their Effect on Parkinson’s Disease
Published: 2021-02-09
Journal: ACS Biomaterials Science & Engineering
Application: WB
Impact Factor: 4.75
Species: Rat
PMID: 33560819
Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model
Published: 2017-01-01
Journal: Theranostics
Application: WB
Impact Factor: 8.77
Species: Mouse
PMID: 28042339
Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury
Published: 2015-02-15
Journal: Purinergic Signalling
Application: IF
Impact Factor: 3.89
Species: Rat
PMID: 25682129
A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells
Published: 2015-02-04
Journal: MOLECULES AND CELLS
Application: WB
Impact Factor: 2.09
Species: Rat
PMID: 25666351
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Product Promise
We promise Anti-p75 NGF Receptor/NGFR Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01187
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026