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- Table of Contents
NGFR antibody (M01187-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of NGFR using anti-NGFR antibody (M01187-1).
NGFR was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NGFR Antibody (M01187-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NGFR was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NGFR Antibody (M01187-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NGFR/P75 Rabbit Monoclonal Antibody
NGFR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
NGFR antibody was positively detected by IHC in:
human glioma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:10000-20000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Human
Recommend
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-NGFR/P75 Rabbit Monoclonal Antibody
View all NGFR Antibodies
View all NGFR Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p75 NGF Receptor
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
45.2 kDa
Cite This Product
Anti-NGFR/P75 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01187-1)
Protocols
Immunohistochemistry (IHC) protocol for NGFR antibody
Publications
Application:
Species:
TLN1 interacts with NGFR and suppresses the development of castration-resistant prostate cancer by upregulating NGFR
Published: 2026-04-23
Journal: Frontiers in Immunology
Application: WB
Impact Factor: 5.90
Species: Human
PMID: 42112334
Olfactory ensheathing cells transplantation alleviates cancer pain by inhibiting P2X7 receptor expression mediated activation of microglia
Published: 2025-07-09
Journal: International Journal of Surgery
Application: IF
Impact Factor: 10.10
Species: Rat
PMID: 40638362
Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation
Published: 2025-05-20
Journal: JOURNAL OF BIOMATERIALS APPLICATIONS
Application: IF
Impact Factor: 2.50
Species: Mouse
PMID: 40391599
Review
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Product Promise
We promise Anti-NGFR/P75 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01187-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026