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Product Info Summary
|Description:||Transient overexpression lysate of translocase of outer mitochondrial membrane 40 homolog (yeast)-like (TOMM40L), nuclear gene encoding mitochondrial protein|
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TOMM40L (NM_032174) Human Over-expression Lysate
Transient overexpression lysate of translocase of outer mitochondrial membrane 40 homolog (yeast)-like (TOMM40L), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
TOMM40L (NM_032174) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084134)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TOMM40L Human Over-expression Lysates NM_032174.
Western blot validation of overexpression lysate (LS084134) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TOMM40L (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TOMM40L (Source: Uniprot.org, NCBI)
Mitochondrial import receptor subunit TOM40B
FLJ12770; mitochondrial import receptor subunit TOM40B; Protein TOMM40-like; TOMM40BRP11-297K8.10; translocase of outer mitochondrial membrane 40 homolog (yeast)-like; translocase of outer mitochondrial membrane 40 homolog-like (yeast); translocase of outer mitochondrial membrane 40 homolog-like TOMM40L TOMM40B translocase of outer mitochondrial membrane 40 like mitochondrial import receptor subunit TOM40B|protein TOMM40-like|translocase of outer mitochondrial membrane 40 homolog-like*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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