TSC22 domain family, member 4 (TSC22D4) (NM_030935) Human Over-expression Lysate

Lysate of Tsc22d4 over-expression cell lines, WB control

Transient overexpression lysate of TSC22 domain family, member 4 (TSC22D4)

Product Info Summary

SKU: LS081628
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of TSC22 domain family, member 4 (TSC22D4)

Product Name

TSC22 domain family, member 4 (TSC22D4) (NM_030935) Human Over-expression Lysate

See all Tsc22d4 products

SKU/Catalog Number

LS081628

Size

100 µg

Description

Transient overexpression lysate of TSC22 domain family, member 4 (TSC22D4)

Storage & Handling

Cite This Product

TSC22 domain family, member 4 (TSC22D4) (NM_030935) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081628)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for Tsc22d4 (Source: Uniprot.org, NCBI)

Gene Name

Tsc22d4

Full Name

TSC22 domain family protein 4

Weight

39.979kDa

Superfamily

TSC-22/Dip/Bun family

Alternative Names

THG1; TILZ2THG-1; TSC22 domain family 4; TSC22 domain family protein 4; TSC22 domain family, member 4; Tsc-22-like protein THG-1; TSC22-related-inducible leucine zipper protein 2 Tsc22d4|0610009M14Rik, 1700023B23Rik, AI415410, Thg-1p, Thg-1pit, Tilz2|TSC22 domain family, member 4|TSC22 domain family protein 4|TSC22 domain family 4|TSC22-related inducible leucine zipper 2|TSC22-related-inducible leucine zipper protein 2

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on Tsc22d4, check out the Tsc22d4 Infographic

Tsc22d4 infographic

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