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Product Info Summary
| SKU: | LS007307 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of U2 small nuclear RNA auxiliary factor 1 (U2AF1), transcript variant a |
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Product info
Product Name
U2AF35 (U2AF1) (NM_006758) Human Over-expression Lysate
SKU/Catalog Number
LS007307
Size
100 µg
Description
Transient overexpression lysate of U2 small nuclear RNA auxiliary factor 1 (U2AF1), transcript variant a
Storage & Handling
Cite This Product
U2AF35 (U2AF1) (NM_006758) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007307)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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U2AF35 (U2AF1) Human Over-expression Lysates NM_006758.
Western blot validation of overexpression lysate (LS007307) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with U2AF1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for U2AF1 (Source: Uniprot.org, NCBI)
Gene Name
U2AF1
Full Name
Splicing factor U2AF 35 kDa subunit
Weight
27.872kDa
Superfamily
splicing factor SR family
Alternative Names
FP793; human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712; RN; RNU2AF1,35-KD subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit U2AF1 FP793, RN, RNU2AF1, U2AF35, U2AFBP U2 small nuclear RNA auxiliary factor 1 splicing factor U2AF 35 kDa subunit|U2 small nuclear RNA auxillary factor 1|U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit|U2 snRNP auxiliary factor small subunit|U2(RNU2) small nuclear RNA auxiliary factor binding protein|splicing factor U2AF 35kDa subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on U2AF1, check out the U2AF1 Infographic
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In this infographic, you will see the following information for U2AF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For U2AF35 (U2AF1) (NM_006758) Human Over-expression Lysate (LS007307)
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