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Product Info Summary
|Description:||Transient overexpression lysate of unc-93 homolog B1 (C. elegans) (UNC93B1)|
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UNC93B (UNC93B1) (NM_030930) Human Over-expression Lysate
Transient overexpression lysate of unc-93 homolog B1 (C. elegans) (UNC93B1)
Storage & Handling
Cite This Product
UNC93B (UNC93B1) (NM_030930) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081622)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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UNC93B (UNC93B1) Human Over-expression Lysates NM_030930.
Western blot validation of overexpression lysate (LS081622) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UNC93B1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for UNC93B1 (Source: Uniprot.org, NCBI)
Protein unc-93 homolog B1
hUNC93B1; MGC126617; unc93 (C. elegans) homolog B1; unc93 homolog B; unc-93 homolog B1 (C. elegans); unc93 homolog B1; unc-93 related protein; UNC93B; Unc-93B1; UNC93protein unc-93 homolog B1 UNC93B1 IIAE1, UNC93, UNC93B, Unc-93B1 unc-93 homolog B1, TLR signaling regulator protein unc-93 homolog B1|unc-93 related protein|unc93 homolog B1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on UNC93B1, check out the UNC93B1 Infographic
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